Scientists of the All-Russian Plant Quarantine Center (FSBI "VNIIKR"), subordinate to Rosselkhoznadzor, have completed work on the development and implementation of methodological recommendations (MR) for the detection and identification of Potato black ringspot nepovirus. The scientific work was carried out in accordance with the provisions of the International Plant Protection Convention and the International Standard for Phytosanitary Measures No. 27.
Currently, the potato black ring spot virus (PBRSV) is included in the A1 lists of quarantine objects of the Russian Federation, the EAEU and the EPPO.
Potato (Solanum tuberosum) is the primary host for potato black ring spot virus.
Potatoes (Solanum tuberosum) are a valuable crop widely grown in our country. In the Russian Federation, potatoes are grown both on commercial plantations on farms of various forms of ownership, and everywhere - on personal and summer cottages.
As an intracellular pathogen, PBRSV can acclimatize in all regions in which its host plant is capable of growing. It is known that PBRSV can be introduced with infected seed potatoes.
The most important measure to prevent the spread of PBRSV is its early diagnosis in potato seed and ware potatoes using modern laboratory research methods, to which these guidelines are devoted.
In the course of preparing methodological recommendations, the scientists of the FGBI "VNIIKR" updated and generalized information about the quarantine object - potato black ring spot virus (PBRSV): nomenclature, host plants, prevalence, biology (methods of transfer and spread), harmfulness and global diagnostic experience.
Based on the results of the studies, a method was developed for the detection and identification of potato black ring spot nepovirus, which provides for a screening test by enzyme immunoassay or "real time" PCR and confirmatory tests by classical PCR.
During the research, scientific monitoring of the territories was carried out, as a result of which 315 potato samples were taken. Scientific monitoring was carried out by visual method based on symptoms caused by the virus.
All proposed laboratory diagnostic methods have been validated. No false positive results were received.
These guidelines are in accordance with laboratory regulations and can be used in the practice of officials and specialists working in the field of plant quarantine when establishing the quarantine phytosanitary state of objects and / or products.